IFNB1 and viral infectious disease: As the Hec1a-IFNB-Luc reporter cell line detects viral dsRNA production at the early stages of viral replication, there is no need for the development of CPE, and viral infection was reliably detected for all viruses tested after a 3-day incubation, which could conceivably be shortened for select more rapidly replicating flaviviruses such as YFV, ZIKV, or TBEV.