Results showed that all of the five m7G regulator diagnostic markers (CYFIP1, LARP1, NUDT4, NUDT16, and DCP2) were differentially expressed among the three groups (Additional file 1: Fig. S3), which further indicated that the five-gene m7G regulator diagnostic signature was effective to discriminate HF subgroups compared to NFDs. This evidence concerns the gene NUDT16 and hydrops fetalis.