The results of GSEA enrichment in this study demonstrated that dasabuvir did affect the cell cycle signaling pathway in ESCC (Fig. 4B) and CDK4 was enriched from proteomics (Fig. 4C–E), Western blotting showed that dasabuvir suppressed the protein levels of CDK4 and cyclin D1 (Fig. 4F) and cell cycle assay also indicated ESCC cells were blocked in the G0/G1 phase (Fig. 4G). Here, CDK4 is linked to esophageal squamous cell carcinoma.