We first confirmed that cuprizone induced similar levels of demyelination and gliosis in the Myrf-Het;Nestin-tdT and the Myrf-cKO;Nestin-tdT mice based on MBP and Eriochrome Cyanine stainings, and on GFAP and Iba1 stainings (Figure 2). This evidence concerns the gene GFAP and Gliosis.