The TGF-β signaling pathway, WNT signaling pathway, Notch signaling pathway, and PI3K signaling pathway were all significantly enriched in the high-enrichment score group, with the TGF-β signaling pathway having the largest NES (|NES| = 2.0875), and we believed that TGF-β signaling played an important role in inducing EMT phenotypes in gliomas (Figures 5K−O). This evidence concerns the gene PIK3CA and central nervous system cancer.