Previous studies suggested that DHA, the precursor of PDX, when used at 30 μM not only protected against palmitate (500 μM)-induced cytotoxicity but also prevented insulin resistance in C2C12 myotubes by decreasing protein kinase C (PKC)-θ activation and restoring cellular acylcarnitine profile, insulin-dependent AKT phosphorylation and glucose uptake. The gene discussed is AKT1; the disease is Insulin resistance.