In this study, we evaluate the performance of the DFAC developed using Cetuximab (an EGFR targeted antibody) conjugated to Benzoporphyrin derivative (BPD) for fluorescence imaging and PIT, and a Si-centered naphthalocyanine (SiNC(OH)) [19] dye for photoacoustic imaging in microscopic head and neck cancer spheroids, in vitro. This evidence concerns the gene EGFR and head and neck cancer.