TWIST1 and posterior cortical atrophy: We also found that collection of OBCM under a prolonged period of time (OBCM-L; the same amount of collection time as for PCa-OBCM) did not further increase N-cadherin or Twist expression, or inhibit E-cadherin expression when compared with OBCM-induced values, suggesting that OBCM-induced EMT activation is limited in PCa cells (Supplementary Fig. 1C).