NFKB1 and central nervous system cancer: As shown, in both shGαi2 and koGαi2 P1 glioma cells (see Figure 3 and 4), phosphorylated p65 levels were robustly decreased (Figure 6F), the NFκB (p65) DNA-binding activity was also reduced in Gαi2-depleted P1 glioma cells (Figure 6G).