To examine whether FOXQ1 upregulation is required for FGFR1 signaling-promoted breast tumor growth in vivo, we injected control DCIS-iFGFR1 cells with sh-NC expression and FOXQ1-knockdown DCIS-iFGFR1 cells with sh-FOXQ1-1/2 expression into the mammary gland fat pads of female BALB/c-nu mice. The gene discussed is FGFR1; the disease is ductal breast carcinoma in situ.