At 6 weeks and 8 weeks after the anti-NgR DNA vaccination, the sera from vaccinated rats were collected for antibody detection and the lysates of neuroblastoma cells that endogenously express NgR was used to react with the postvaccination sera. Fc region of human IgG1 into the DNA vaccine improved the efficiency of antigen presentation by targeting the antigen to antigen-presenting cells (APCs) because most APCs express Fc receptors. The gene discussed is RTN4R; the disease is neuroblastoma.