Human NgR containing a leading peptide was fused with the Fc fragment derived from human IgG1. The recombinant plasmid pcDNA3.1-hNgR-Fc was identified by restriction endonuclease digestion and confirmed by DNA sequencing analysis followed by vaccination of SCI model where it stimulates the production of anti-NgR antibody to overcome NgR-mediated growth inhibition after spinal cord injury (SCI). This evidence concerns the gene RTN4R and spinal cord injury.