PCK2 and brain ischemia: In addition, a recent study of rats with global cerebral ischemia demonstrated decreases in hepatic expression of mRNA for pyruvate carboxylase (50%), PEPCK (56%), and G6Pase (80%) at 24 h after ischemia, which resulted in profoundly reduced gluconeogenesis in the liver (Sá-Nakanishi et al., 2020).