Among PKs, PKM2 is notably up-regulated in HCC patients, and a high level of PKM2 is associated with a poor prognosis. Silencing of PKM2 inhibits HCC proliferation, migration, and invasion, whereas overexpression of PKM2 increases IFNγ-positive CD8+ T cells in the HCC mouse model by activating the immune checkpoint blockade. This evidence concerns the gene ARAF and hepatocellular carcinoma.