Furthermore, we used flow cytometry analysis to examine liver macrophages (Figure 3B) and found that HFD promoted F4/80+ expression in HMΦs in both Casp11–/– and WT mice (Figures 3C), indicating that HFD-induced NAFLD drives the increase of NASH-related F4/80+ HMΦs in WT mice, which are caspase-11 independent. The gene discussed is SCAF11; the disease is metabolic dysfunction-associated steatohepatitis.