Furthermore, integrating the findings of the three algorithms, we found that NCAPG2 was statistically significant negatively correlated with the infiltration level of immune cells in LUSC, TGCT, SKCM, ESCA, BLCA and other malignancies, driving the formation of an immunosuppressive microenvironment in part. The gene discussed is NCAPG2; the disease is bladder transitional cell carcinoma.