The ability of NCAPG2 to effectively affect the proliferation of PC cells was confirmed by CCK8 assay (Figures 9H, I) and colony formation assay (Figures 10E–H), which showed that NCAPG2 knockdown markedly reduced the proliferation ability of PC cells in PANC1 and MIAPaCa-2 compared to the control group, which was also supported by the colony formation assay. This evidence concerns the gene NCAPG2 and pachyonychia congenita.