CD8A and neoplasm: For example, single-cell sequencing revealed that CD8+T cells with mutation-associated neoantigen specificity expressed lower levels of interleukin-7 receptor in non-small cell lung cancer treated with anti-PD-1, providing clues for overcoming PD-1 resistance (52).Furthermore, single-cell sequencing analysis also found that targeted repair of DNA damagecan coordinate synergistically or cooperatively with immunotherapy to produce effective anti-tumor response by regulating the immune microenvironment (34).