Finally, GSEA functional enrichment analysis was used to predict the function of KNL1 in the development of endometrial carcinoma, and it was found that KNL1 was closely associated with hallmark allograft rejection, hallmark complement, hallmark Kras signaling up, hallmark g2m checkpoint, hallmark mitotic spindle, hallmark mtorc1 signaling, hallmark e2f targets, hallmark myc targets v1 and other pathways, as shown in Figures 4E, F. The gene discussed is KNL1; the disease is endometrial carcinoma.