To further verify the specificity of nimesulide, we tested its effect on expression of death receptor 4 (DR4), which is a homologous member of the TNF receptor superfamily (51% identical and 68% like DR5) that binds to TRAIL and triggers apoptosis.51,52 We tested the expression of DR4 in all three cancer cell lines in the presence and absence of nimesulide using a western blot. This evidence concerns the gene TNFSF10 and cancer.