FBXO22 and acute myeloid leukemia: We performed a limiting dilution assay with sorted GFP+Fbxo22+/+ and Fbxo22–/– AML cells from primary recipients and found that the frequency of LSCs in Fbxo22–/– AML mice (1:97,540 cells) was significantly lower compared with the Fbxo22+/+ mice (1:223 cells) (Fig. 4G).