To further examine the potential role of FBXO22 in MLLr AML, we knocked down FBXO22 expression in MLLr AML cell line THP-1 (Fig. 1F) and found that FBXO22 knockdown resulted in a markedly inhibition of cell growth and clonogenicity in THP-1 cells (Fig. 1G, H). This evidence concerns the gene FBXO22 and acute myeloid leukemia.