Immunohistochemical (IHC) analysis of ERβ protein expression in the pre-tamoxifen treatment brain tumor specimen showed weak expression of the protein (Fig. 2A) and analysis of ERβ-mutant p53 interaction in the tumor specimen by proximity ligation assay (PLA) demonstrated no interaction between the 2 proteins Fig. 2C). The gene discussed is TP53; the disease is brain neoplasm.