To investigate the effect of leptin on the pro-inflammatory cytokines, we used an enzyme-linked immunosorbent assay (ELISA) to assess the secretion of pro-inflammatory cytokines in immortalized human uterine leiomyoma (HuLM) cells and uterine smooth muscle cells (UtSM) media after treatment with leptin. Here, LEP is linked to Uterine leiomyoma.