Induction of apoptosis was reported by Abdullah et al. in HER2 positive (SK-BR-3) and triple-negative (MDA-MB-231) breast cancer cell lines via PI3K/AKT/FOXO3a pathway inhibition treated with eugenol in a dose-dependent manner that led to the significant increase in the protein levels of AKT serine/threonine kinase 1 (AKT), forkhead box O3 (FOXO3a), cyclin-dependent kinase inhibitor (p27), caspase-3 and -9, and cyclin dependent kinase inhibitor 1A (p21). This evidence concerns the gene CDKN1A and breast carcinoma.