The current study aimed to investigate whether lutein induces apoptosis through NADPH oxidase-mediated ROS production and subsequent NF-κB activation in gastric cancer AGS cells, by evaluating cell viability, colony formation, and apoptotic indices (Bax, Bcl-2, caspase-3 activation, and DNA fragmentation). The gene discussed is NFKB1; the disease is gastric cancer.