Furthermore, a previous study showed that global NOX2 deletion mice increased activity of NADPH oxidase isoforms contributes to worsening outcomes following stroke, at least in males [94], and administration of apocynin before cerebral ischaemia improves outcome in wild-type, but not NOX2-deficient mice, suggesting apocynin’s protective effects occur via NOX2 inhibition (Figure 1) [94]. This evidence concerns the gene CYBB and stroke disorder.