Therefore, to determine the precise molecular mechanism involved in inhibiting the proliferation of breast cancer cell lines, MCF-7, MDA-MB-231, and MDA-MB-453 were treated with SKACP003 at their respective IC50 concentrations for 24 h, and the corresponding expression of CDK-4 and CDK-6 genes was evaluated. The gene discussed is CDK6; the disease is breast carcinoma.