The analysis of tumor-associated immune cells through quantification of the cytotoxic (CD3+CD8+) and regulatory (Treg- CD4+CD25+FoxP3+) T cells, myeloid-derived suppressor cells (MDSCs- Gr-1+CD11b+), and M2 macrophages (CD206+) in melanoma tissue demonstrated the clear distinction between control and treated groups. The gene discussed is CD4; the disease is melanoma.