The cells from the prostate tissue, PCCs, and PDOs were assessed for the epithelial basal (CK5, p63) and luminal (CK18, AR1) markers, mesenchymal (vimentin) marker, and cancer (AMACR, TMPRSS2-ERG, and histone methyltransferase EZH2) markers by RT-PCR. The gene discussed is TMPRSS2; the disease is cancer.