The present study was initiated to determine the stability of seven genes (Gapdh, Actb, 18S rRNA, Hprt1, Hmbs, Ywhaz, and Bud23) using qRT-PCR to establish their suitability as housekeeping genes for an in vivo cerebral ischemia and reperfusion model in gerbils. This evidence concerns the gene ACTB and brain ischemia.