In HCC stem-like cells, both GLS1 and Glutamate–Cysteine Ligase Catalytic Subunit (GCLC), the rate-limiting enzyme of GSH synthesis, are highly expressed, and associated with expression of multiple stem cell markers such as KFL4, SOX2, Nanog, Oct4, CD13 and CD44. Here, GLS is linked to hepatocellular carcinoma.