To better understand the effects of dietary MOS and bacterial infection on the dynamic expression patterns of tight junction-related proteins and transcriptional regulators, western blot was performed and the protein levels of M. amblycephala claudin 3, occludin, ZO-1, p38, PKCα and NF-κB p65 in the intestines are shown in Figure 9. The gene discussed is NFKB1; the disease is bacterial infectious disease.