GNPAT and Myocardial fibrosis: In order to investigate whether myocardial fibrosis resulting in hypertrophy of the LV could play a role in the cardiac phenotype of ether-lipid-deficient mice, we applied several histological staining techniques (H&E, Masson–Goldner trichrome, Sirius Red, and van Gieson) on cardiac tissue from middle-aged (13 months) WT and Gnpat KO mice.