Therefore, the major objective of this study was to characterize three subsets of monocytes from glioma patients’ peripheral blood (PB), with particular emphasis on immune checkpoints (PD-1 (programmed cell death 1 protein), PD-L1, SIRPalpha (signal-regulatory protein alpha), TIM-3 (T cell Ig and mucin domain 3), chemokine receptors (CCR2, CCR5, CX3CR1) expression, as well as pro- (TNF, IL-12) and anti-inflammatory (TGF-beta, IL-10) cytokines’ intracellular profile. The gene discussed is SIRPA; the disease is glioma.