SPAST and glioblastoma: In order to understand if inadequate phosphorylation of the overexpressed Spastin protein in the cells was responsible for the lack of this interaction, we performed the pull-down assay by triggering the phosphorylation of the exogenously overexpressed Spastin and investigated the interaction of exogenous SpastinM1&M87 with Pin1 upon EGF treatment based on the observed effect of EGF administration on the localization of Spastin in T98G glioblastoma cells (Figure 3).