These soluble mediators along with various growth factors such as EGF, fibroblast growth factor (FGF), hepatocyte growth factor (HGF), platelet-derived growth factor (PDGF), tumour growth factor (TGF)-β, and vascular endothelial growth factor (VEGF) along with immune cells in the TME such as the immune infiltrating macrophages, CAFs, neutrophils, and platelets can promote EMT in primary tumours. This evidence concerns the gene VEGFA and neoplasm.