GPCR expression in human subcutaneous adipose tissue and the impact of obesity RNA sequencing detected a total of 257 GPCRs in subcutaneous adipose of healthy human subjects, of which 172 were expressed above trace levels, 85 were expressed at trace levels whereas 99 GPCRs were absent or not detected (Figure 5A). This evidence concerns the gene FZD4 and obesity due to melanocortin 4 receptor deficiency.