FDX1 and cancer: To further analyze the correlation of aberrant FDX1 expression with methylation and RNA modification, we first calculated the correlation index of methylation site beta values and FDX1 expression, which indicated that DNA methylation played a negative regulatory role in most cancer types, including ACC, BRCA, CHOL, ESCA, HNSC, KICH, KIRP, LIHC, LUAD, OV, PCPG, SKCM, TGCT, and UCEC (Figure 3A).