In HCC cells with FBXO43 knockdown, ectopic expression of UBE2C re-decreased the level of p53, which could be recovered by ectopic expression of p53 again, as represented in Figure 6A. Therefore, the findings of functional experiments such as CCK-8, plate clone formation, and transwell invasion assays revealed that UBE2C overexpression could significantly remove the inhibitory effects of FBXO43 depletion on cell proliferation and invasion, which were re-suppressed by co-overexpression of p53 and UBE2C in HCC cells with FBXO43 knockdown (Figure 6B–D). Here, TP53 is linked to hepatocellular carcinoma.