POU5F1 and hepatocellular carcinoma: Additionally, the sorafenib-resistant HCC cells exhibited more favorable stemness-related properties, including stemness-related genes (OCT4, NANOG, SOX2) and EMT-related genes (CD44, N-CAD, Vimentin) (Figure 1D), secondary sphere formation ability (Figure 1E), and cell migration ability (Figure 1F).