ESR2 and pancreatic adenocarcinoma: Figure 6B,C show the most significantly enriched pathways in ESR1-high and ESR2-high groups, including the B-cell receptor signaling pathway, mature B-cell differentiation, T-cell migration, positive T-cell selection, T-helper 17 type immune response, etc. Since B-cells and T-cells were the predominant cell types in TLS structures, the results identified significantly up-regulated T/B-cell-related pathways in PAAD with high ERs expression, which further demonstrated the potential stimulatory role of ER signaling in PAAD-associated TLS formation and function.