The human epidermoid carcinoma cell line A431, the EGFR-mutated non-small cell lung cancer (NSCLC) cell line HCC827, and the estrogen receptor-positive human breast cancer cell line MCF7 transfected with human epidermal growth factor 2 (HER2/NEU, MCF7-HER2), [33,35,36,37,38,39] were cultured in DMEM 1× (Gibco/Thermo Fisher Scientific) with 10% fetal bovine serum (FBS, Seradigm, Sanborn, NY, USA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific). The gene discussed is ESR1; the disease is non-small cell lung carcinoma.