Our data demonstrate the presence of stable chromatin loops in the loci encoding for of DAPK1, HSD3B2, SRD5A3, MMP1, and miRNA98. Together with PSA taken as a continuous variable this epigenetic test has yielded a combined PPV of 0.92 and a NPV of 0.94 for PCa diagnosis in a mixed cohort with cancer prevalence of 34%. The gene discussed is KLK3; the disease is cancer.