CASP3 and osteosarcoma: Figure 8A,B showed that treatment with ononin increased the rate of tumor cell death in MG-63 cells compared to the control group. Quantitative analysis of data also showed that incubation with ononin increased both the early and late stages of apoptotic osteosarcoma when compared to the control group (Figure 8A,B). The results were in line with data on caspase 3/7 protein expression, which confirmed that ononin treatment reduced the activation of caspase-3/7 (Figure 8C,D).