To further rule out any confounding effects of the sgRNA library as well as off-targeting effects, we transduced Pik3caH1047R;LSL-Cas9-GFP mice with an independent Elovl4 sgRNA, which corroborated our findings and established Elovl4 as a novel SCC tumor suppressor (Figure 3E,F). The gene discussed is ELOVL4; the disease is neoplasm.