CD27 and colorectal carcinoma: As shown in Figure 2A,B (polychromatic immunofluorescence assay and flow cytometry analysis on Vδ2 gated cells, see Figure S4), the majority of Vδ2 T lymphocytes expanded in co-culture with CRC-TAF exposed to ZA, displayed the phenotype of EM cells (>45% CD45RA− CD27−) and of TEMRA (CD45RA+CD27−).