Of note, the cultured CRC-TAF cell lines 16-004, 16-016, 16-035 expressed BTN2A1 on their surface and in the cytoplasm, evidenced by immunofluorescence and flow cytometry (Figure 5E,F), whereas BTN3A1 membrane and cytoplasmic expression was less detectable (Figure 5E,F). Here, BTN2A1 is linked to colorectal carcinoma.