Several proteins identified in the 100k EVs have already been characterized as possible vaccine candidates against fascioliasis, including cathepsin L [28], leucine aminopeptidase [29], 14-3-3 [30], fatty acid binding protein (FABP) [31], phosphoglycerate kinase [32], cathepsin B [33], and tetraspanin 2 (-TSP2) [34], but with different protective efficacies. This evidence concerns the gene TSPAN2 and fascioliasis.