Taking the relevant factors affecting the baseline balance (Additional file 1: Table S3) and the factors that may affect prognosis in the univariate analysis as confounding factors into the Cox multivariate model (p < 0.1, Additional file 1: Table S4), the expression level of TLR9 was found to be an independent prognostic factor of B-ALL (Fig. 1B), which is related to progression free survival (PFS) (mPFS: 29.0 months vs. 9.0 months, p = 0.005, HR: 8.091) and overall survival (OS) (mOS: undefined vs. 16.0 months, p = 0.018, HR: 5.799). The gene discussed is TLR9; the disease is precursor B-cell acute lymphoblastic leukemia.