To explore the molecular mechanism of the kidney injury induced by the GO administration, we extended the detection to mRNA expression levels of two AKI biomarkers: KIM1 and NGAL, and several important inflammatory cytokines, including Ccl2 (also known as Mcp1), Tnfα, IL1β and IL6, in the high dose (15 mg/kg) of GO treated kidney tissue lysates. The gene discussed is TNF; the disease is acute kidney injury.