Molecular analyses based on NGS were performed at Xiamen Spacegen Co., Ltd including a 11-gene panel (POLE, TP53, PTEN, MSH2, MSH6, MLH1, PMS2, EPCAM, KRAS, PIK3CA, CTNNB1) designed for endometrial molecular classification, microsatellite instability (MSI) testing containing 34 loci and a 602-gene panel (2.68 Mb) for tumor mutation burden analysis. The gene discussed is MLH1; the disease is neoplasm.