While, the levels of TNF-α and TNF-β was slightly changed between patients with primary gastrointestinal cancers and healthy donors, but there were no statistical significances (Fig. 7D, E), These results suggested that the apoptosis and secreted cytokines of immune cells were affected by the mitochondrial dysfunction in the primary gastrointestinal cancers without anti-tumor treatments. This evidence concerns the gene LTA and neoplasm.