Single-nucleus genotype heatmaps and Venn diagrams (Fig. 4b–e) of multiregional samples PR02-3 and PR02-4 suggested colocalization of the major KRAS p.G12D with another likely driver TP53 p.C203Y, which signified the major tumor clone in this sample; the minor KRAS p.G12V -bearing clone was mutually exclusive with the above two drivers and did not colocalize with any known driver gene mutation at similar clonal frequency. This evidence concerns the gene KRAS and neoplasm.