TP53 and neoplasm: Single-nucleus genotype heatmaps and Venn diagrams (Fig. 4b–e) of multiregional samples PR02-3 and PR02-4 suggested colocalization of the major KRAS p.G12D with another likely driver TP53 p.C203Y, which signified the major tumor clone in this sample; the minor KRAS p.G12V -bearing clone was mutually exclusive with the above two drivers and did not colocalize with any known driver gene mutation at similar clonal frequency.